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Isolation and characterization of a human cementocyte-like cell line, HCY-23.
- Source :
-
Brazilian oral research [Braz Oral Res] 2019 Aug 15; Vol. 33, pp. e058. Date of Electronic Publication: 2019 Aug 15. - Publication Year :
- 2019
-
Abstract
- Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.
- Subjects :
- Adaptor Proteins, Signal Transducing
Adolescent
Adult
Analysis of Variance
Bone Morphogenetic Proteins analysis
Bone Morphogenetic Proteins genetics
Cell Line
Dental Cementum metabolism
Extracellular Matrix Proteins analysis
Extracellular Matrix Proteins genetics
Female
Fluorescent Antibody Technique
Gene Expression
Genetic Markers genetics
Humans
Male
Membrane Glycoproteins analysis
Membrane Glycoproteins genetics
Molar cytology
Phosphates pharmacology
Phosphoproteins analysis
Phosphoproteins genetics
Sialoglycoproteins analysis
Sialoglycoproteins genetics
Time Factors
Young Adult
Cell Culture Techniques methods
Dental Cementum cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1807-3107
- Volume :
- 33
- Database :
- MEDLINE
- Journal :
- Brazilian oral research
- Publication Type :
- Academic Journal
- Accession number :
- 31432925
- Full Text :
- https://doi.org/10.1590/1807-3107bor-2019.vol33.0058