Back to Search
Start Over
Investigation of fatty aldehyde and alcohol synthesis from fatty acids by αDox- or CAR-expressing Escherichia coli.
- Source :
-
Journal of biotechnology [J Biotechnol] 2019 Nov 10; Vol. 305, pp. 11-17. Date of Electronic Publication: 2019 Aug 17. - Publication Year :
- 2019
-
Abstract
- Fatty aldehydes are among the most important flavor and fragrance compounds. Most biotechnological production approaches make use of the one step conversion of fatty acids from renewable sources by the enzymes α-dioxygenase (αDox) or carboxylic acid reductase (CAR). Their reaction mechanisms and cofactor dependencies are very different. In contrast to heme-containing αDox which requires only oxygen as cosubstrate, CAR needs NADPH and ATP, which is a clear argument for the application of a whole cell catalyst. Therefore we compared fatty acid biotransformations with growing Escherichia coli cells expressing αDox or CAR to investigate their suitability for fatty aldehyde and also fatty alcohol production. Our results show the main product of fatty acid conversions with αDox-expressing cells to be the expected C <subscript>n-1</subscript> aldehyde. However, 14% of the products consist of the corresponding alcohol, but in addition, 17% of the products consist of further shortened aldehydes, alcohols and acids that result from the consecutive activity of αDox and a putative endogenous fatty aldehyde dehydrogenase activity in E. coli. Conversely, CAR-expressing cells produced only the unshortened fatty aldehyde and alcohol, whereby the latter surprisingly accounts for at least 80% of the products. The considerably higher extend of aldehyde reduction of CAR-expressing cells was shown to be causally connected to the CAR-mediated fatty acid conversion. Our study provides an overview about the applicability of αDox- or CAR-based whole cell catalysts and gives a detailed description of side products as well as suggestions for tailored strain engineering.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Adenosine Triphosphate metabolism
Aldehydes
Catalysis
Dioxygenases genetics
Escherichia coli genetics
Escherichia coli metabolism
Fatty Acids metabolism
Genetic Engineering
NADP metabolism
Oryza enzymology
Oryza genetics
Oxidation-Reduction
Oxidoreductases genetics
Dioxygenases metabolism
Escherichia coli growth & development
Fatty Acids biosynthesis
Fatty Alcohols metabolism
Oxidoreductases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4863
- Volume :
- 305
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 31430497
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2019.08.011