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Trans,trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties.
- Source :
-
PloS one [PLoS One] 2019 Aug 20; Vol. 14 (8), pp. e0220718. Date of Electronic Publication: 2019 Aug 20 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farnesol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Biofilms drug effects
Biofilms growth & development
Hardness
Humans
Materials Testing
Microbial Viability drug effects
Streptococcus mutans drug effects
Streptococcus mutans physiology
Tensile Strength
Anti-Bacterial Agents chemistry
Anti-Bacterial Agents pharmacology
Farnesol analogs & derivatives
Farnesol pharmacology
Glass Ionomer Cements chemistry
Glass Ionomer Cements pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 14
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 31430298
- Full Text :
- https://doi.org/10.1371/journal.pone.0220718