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Serum miR-1301-3p, miR-335-5p, miR-28-5p, and their target B7-H3 may serve as novel biomarkers for colorectal cancer.
- Source :
-
Journal of B.U.ON. : official journal of the Balkan Union of Oncology [J BUON] 2019 May-Jun; Vol. 24 (3), pp. 1120-1127. - Publication Year :
- 2019
-
Abstract
- Purpose: B7-H3, a member of the B7 family of immune regulatory ligands, plays a critical role in the T cell-mediated immune response. It is broadly expressed in several human cancers and leads to poor prognosis. Nevertheless, the clinical significance of B7-H3 expression in colorectal cancer (CRC) remains unclear.<br />Methods: The serum B7-H3, B7-H1, cancer-associated carbohydrate antigen-50 (CA-50) and carcinoembryonic antigen (CEA) expressions in patients with CRC, benign gastrointestinal diseases, and healthy controls were measured by ELISA. The miRNAs that target B7-H3 were predicted by using the miRTarBase. Real-time PCR was performed to examine their expressions in patients with CRC.<br />Results: B7-H3, B7-H1, and CA-50 expressions were higher in patients with CRC than those in healthy controls and the patients with benign gastrointestinal diseases. B7-H3 expression was correlated with TNM stage and metastasis. It was predicted that B7-H3 was a target of miR-1301-3p, miR-335-5p, and miR-28-5p and its expression was negatively related to these three miRNAs expressions. Serum miR-1301-3p, miR-335-5p, and miR-28-5p expressions were also correlated with the TNM stage and metastasis.<br />Conclusion: Our results indicated that serum miR-1301-3p, miR-28-5p, miR-335-5p, and B7-H3 expressions were correlated with pathological stages of CRC and metastasis and may therefore serve as novel biomarkers for CRC diagnosis and treatment.
Details
- Language :
- English
- ISSN :
- 2241-6293
- Volume :
- 24
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of B.U.ON. : official journal of the Balkan Union of Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 31424670