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Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential.
- Source :
-
Developmental cell [Dev Cell] 2019 Oct 07; Vol. 51 (1), pp. 35-48.e7. Date of Electronic Publication: 2019 Aug 15. - Publication Year :
- 2019
-
Abstract
- Centromeres provide a robust model for epigenetic inheritance as they are specified by sequence-independent mechanisms involving the histone H3-variant centromere protein A (CENP-A). Prevailing models indicate that the high intrinsic stability of CENP-A nucleosomes maintains centromere identity indefinitely. Here, we demonstrate that CENP-A is not stable at centromeres but is instead gradually and continuously incorporated in quiescent cells including G0-arrested tissue culture cells and prophase I-arrested oocytes. Quiescent CENP-A incorporation involves the canonical CENP-A deposition machinery but displays distinct requirements from cell cycle-dependent deposition. We demonstrate that Plk1 is required specifically for G1 CENP-A deposition, whereas transcription promotes CENP-A incorporation in quiescent oocytes. Preventing CENP-A deposition during quiescence results in significantly reduced CENP-A levels and perturbs chromosome segregation following the resumption of cell division. In contrast to quiescent cells, terminally differentiated cells fail to maintain CENP-A levels. Our work reveals that quiescent cells actively maintain centromere identity providing an indicator of proliferative potential.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Cycle
Cell Cycle Proteins metabolism
Cell Differentiation
Cell Division
Cell Line
Cell Proliferation
Centromere ultrastructure
Epigenesis, Genetic
Female
Green Fluorescent Proteins metabolism
Humans
Male
Meiosis
Mice
Mice, Inbred C57BL
Oocytes metabolism
Protein Serine-Threonine Kinases metabolism
Proto-Oncogene Proteins metabolism
RNA, Small Interfering metabolism
Starfish metabolism
Testis metabolism
Polo-Like Kinase 1
Centromere metabolism
Centromere Protein A metabolism
Muscle, Skeletal metabolism
Nucleosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1551
- Volume :
- 51
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental cell
- Publication Type :
- Academic Journal
- Accession number :
- 31422918
- Full Text :
- https://doi.org/10.1016/j.devcel.2019.07.016