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Heterogeneity of Highly Susceptible Yersinia enterocolitica Isolates of Clinical and Environmental Origin: A 5-Year Survey from Iran (2011-2016).
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2020 Jan; Vol. 26 (1), pp. 46-53. Date of Electronic Publication: 2019 Aug 16. - Publication Year :
- 2020
-
Abstract
- The aim of this study was to evaluate the resistance and virulence characteristics of Yersinia enterocolitica strains of clinical and environmental origins over a 5-year period in Iran and to determine the genetic diversity of strains using pulsed-field gel electrophoresis (PFGE) method. A total of 20 Y. enterocolitica strains were collected from 850 stool samples of patients with diarrhea, and 18 Y e rsinia spp. including 10 Y. enterocolitica were collected from water, food, and vegetable samples. The most frequently isolated Y. enterocolitica strains belonged to biotype (BT) 1A (83.33%). No Y. enterocolitica BT4 was detected that can be attributed to the absence of pig animal reservoir in Iranian food chain. The most frequent chromosomal virulence genes among the Y. enterocolitica isolates were inv (100%), ys tA (67%), ystB (83%), tccC (20%), and ail (17%). The most frequent chromosomal virulence genes among non- enterocolitica Yersinia spp. isolates were ystB (87.5%), ystA (37.5%), and inv (37.5%). None of the Y. enterocolitica isolates harbored plasmid origin virulence genes. None of the isolates was resistant to ciprofloxacin, gentamicin, tetracycline, cotrimoxazole, and chloramphenicol, whereas 90% of the Y. enterocolitica and 62.5% of the Yersinia spp. strains were resistant to ampicillin. PFGE genotyping showed a heterogeneous population of highly susceptible Yersinia spp. in both clinical and environmental samples, putting forward a good prognosis in the treatment of patients with yersiniosis. The occurrence of biotype 1A with inv <superscript>+</superscript> ystA <superscript>+</superscript> ystB <superscript>+</superscript> genotype in clinical strains implies the significance of inv , ystA , and ystB gene products in turning of naturally nonpathogenic biotype 1A strains into clinically important pathogens.
- Subjects :
- Diarrhea drug therapy
Diarrhea microbiology
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Genotype
Humans
Iran epidemiology
Virulence genetics
Yersinia Infections drug therapy
Yersinia Infections microbiology
Yersinia enterocolitica drug effects
Yersinia enterocolitica pathogenicity
Anti-Bacterial Agents pharmacology
Diarrhea epidemiology
Yersinia Infections epidemiology
Yersinia enterocolitica isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 26
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 31414976
- Full Text :
- https://doi.org/10.1089/mdr.2018.0469