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Follicular structures of cows with cystic ovarian disease present altered expression of cytokines.
- Source :
-
Zygote (Cambridge, England) [Zygote] 2019 Oct; Vol. 27 (5), pp. 285-298. Date of Electronic Publication: 2019 Aug 15. - Publication Year :
- 2019
-
Abstract
- Ovulation is considered an inflammatory, cytokine-mediated event. Cytokines, which are recognized as growth factors with immunoregulatory properties, are involved in many cellular processes at the ovarian level. In this sense, cytokines affect fertility and are involved in the development of different ovarian disorders such as bovine cystic ovarian disease (COD). Because it has been previously demonstrated that ovarian cells represent both sources and targets of cytokines, the aim of this study was to examine the expression of several cytokines, including IL-1β, IL-1RA, IL-1RI, IL-1RII, IL-4 and IL-8, in ovarian follicular structures from cows with spontaneous COD. The protein expression of these cytokines was evaluated by immunohistochemistry. Additionally, IL-1β, IL-4 and IL-8 concentrations in follicular fluid (FF) and serum were determined by enzyme-linked immunosorbent assay (ELISA). In granulosa and theca cells, IL-1RI, IL-1RII, IL-1RA and IL-4 expression levels were higher in cystic follicles than in the control dominant follicles. The serum and FF concentrations of IL-1β and IL-4 showed no differences between groups, whereas IL-8 concentration was detected only in FF of cysts from cows with COD. The FF and serum concentrations of IL-1β and IL-8 showed no significant differences, whereas IL-4 concentration was higher in FF than in serum in both the control and COD groups. These results evidenced an altered expression of cytokines in ovaries of cows with COD that could contribute to the pathogenesis of this disease.
- Subjects :
- Animals
Case-Control Studies
Cattle
Cattle Diseases
Female
Interleukin 1 Receptor Antagonist Protein blood
Interleukin 1 Receptor Antagonist Protein metabolism
Interleukin-1beta metabolism
Interleukin-4 blood
Interleukin-4 metabolism
Interleukin-8 blood
Interleukin-8 metabolism
Ovarian Cysts veterinary
Ovarian Follicle metabolism
Ovarian Follicle pathology
Receptors, Interleukin-1 Type I metabolism
Receptors, Interleukin-1 Type II metabolism
Follicular Fluid metabolism
Interleukins metabolism
Ovarian Cysts metabolism
Ovarian Cysts pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8730
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zygote (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 31412964
- Full Text :
- https://doi.org/10.1017/S0967199419000285