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Bicoid-Dependent Activation of the Target Gene hunchback Requires a Two-Motif Sequence Code in a Specific Basal Promoter.
- Source :
-
Molecular cell [Mol Cell] 2019 Sep 19; Vol. 75 (6), pp. 1178-1187.e4. Date of Electronic Publication: 2019 Aug 08. - Publication Year :
- 2019
-
Abstract
- In complex genetic loci, individual enhancers interact most often with specific basal promoters. Here we investigate the activation of the Bicoid target gene hunchback (hb), which contains two basal promoters (P1 and P2). Early in embryogenesis, P1 is silent, while P2 is strongly activated. In vivo deletion of P2 does not cause activation of P1, suggesting that P2 contains intrinsic sequence motifs required for activation. We show that a two-motif code (a Zelda binding site plus TATA) is required and sufficient for P2 activation. Zelda sites are present in the promoters of many embryonically expressed genes, but the combination of Zelda plus TATA does not seem to be a general code for early activation or Bicoid-specific activation per se. Because Zelda sites are also found in Bicoid-dependent enhancers, we propose that simultaneous binding to both enhancers and promoters independently synchronizes chromatin accessibility and facilitates correct enhancer-promoter interactions.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
DNA-Binding Proteins genetics
Drosophila Proteins genetics
Drosophila melanogaster
Homeodomain Proteins genetics
Nuclear Proteins genetics
Nuclear Proteins metabolism
Trans-Activators genetics
Transcription Factors genetics
DNA-Binding Proteins biosynthesis
Drosophila Proteins biosynthesis
Drosophila Proteins metabolism
Homeodomain Proteins metabolism
Nucleotide Motifs
Response Elements
Trans-Activators metabolism
Transcription Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 75
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 31402096
- Full Text :
- https://doi.org/10.1016/j.molcel.2019.06.038