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In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 2048, pp. 93-106. - Publication Year :
- 2019
-
Abstract
- In this chapter, we describe a protocol for hematopoietic differentiation of nonhuman primate (NHP)-induced pluripotent stem cells (iPSCs) derived from T cells and generation of T cells. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells, in particular CD8 single-positive (SP) T cells.
- Subjects :
- Animals
Cell Line
Cell Separation instrumentation
Coculture Techniques instrumentation
Coculture Techniques methods
Culture Media metabolism
Cytokines metabolism
Fibroblasts
Flow Cytometry instrumentation
Fluorescent Antibody Technique, Direct instrumentation
Fluorescent Antibody Technique, Direct methods
Hematopoietic Stem Cells
Humans
Mesenchymal Stem Cells
Mice
Primates
CD8-Positive T-Lymphocytes physiology
Cell Differentiation
Cell Separation methods
Flow Cytometry methods
Induced Pluripotent Stem Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2048
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 31396934
- Full Text :
- https://doi.org/10.1007/978-1-4939-9728-2_11