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Force-Dependent Regulation of Talin-KANK1 Complex at Focal Adhesions.
- Source :
-
Nano letters [Nano Lett] 2019 Sep 11; Vol. 19 (9), pp. 5982-5990. Date of Electronic Publication: 2019 Aug 13. - Publication Year :
- 2019
-
Abstract
- KANK proteins mediate cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix. KANKs interact with the integrin/actin-binding protein talin and with several components of microtubule-stabilizing cortical complexes. Because of actomyosin contractility, the talin-KANK complex is likely under mechanical force, and its mechanical stability is expected to be a critical determinant of KANK recruitment to focal adhesions. Here, we quantified the lifetime of the complex of the talin rod domain R7 and the KN domain of KANK1 under shear-force geometry and found that it can withstand forces for seconds to minutes over a physiological force range up to 10 pN. Complex stability measurements combined with cell biological experiments suggest that shear-force stretching promotes KANK1 localization to the periphery of focal adhesions. These results indicate that the talin-KANK1 complex is mechanically strong, enabling it to support the cross-talk between microtubule and actin cytoskeleton at focal adhesions.
- Subjects :
- Actin Cytoskeleton chemistry
Actin Cytoskeleton genetics
Actomyosin chemistry
Actomyosin genetics
Adaptor Proteins, Signal Transducing genetics
Cell Adhesion genetics
Cytoskeletal Proteins genetics
Extracellular Matrix chemistry
Extracellular Matrix genetics
Focal Adhesions genetics
HeLa Cells
Humans
Integrins chemistry
Integrins genetics
Mechanical Phenomena
Mechanotransduction, Cellular genetics
Microtubules chemistry
Microtubules genetics
Multiprotein Complexes genetics
Muscle Contraction genetics
Shear Strength physiology
Talin genetics
Adaptor Proteins, Signal Transducing chemistry
Cytoskeletal Proteins chemistry
Focal Adhesions chemistry
Multiprotein Complexes chemistry
Talin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1530-6992
- Volume :
- 19
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Nano letters
- Publication Type :
- Academic Journal
- Accession number :
- 31389241
- Full Text :
- https://doi.org/10.1021/acs.nanolett.9b01732