Back to Search
Start Over
Single-Cell Phenotypic Profiling of CTCs in Whole Blood Using an Integrated Microfluidic Device.
- Source :
-
Analytical chemistry [Anal Chem] 2019 Sep 03; Vol. 91 (17), pp. 11078-11084. Date of Electronic Publication: 2019 Aug 14. - Publication Year :
- 2019
-
Abstract
- Single-cell phenotypic profiling of circulating tumor cells (CTCs) in the blood of cancer patients can reveal vital tumor biology information. Even though various approaches have been provided to enrich and detect CTCs, it remains challenging for consecutive CTC sorting, enumeration, and single-cell characterizations. Here, we report an integrated microfluidic device (IMD) for single-cell phenotypic profiling of CTCs that enables automated CTCs sorting from whole blood following continuous single-cell phenotypic analysis while satisfying the requirements of both high purity (92 ± 3%) of cell sorting and high-throughput processing capacity (5 mL whole blood/3 h). Using this new technique we test the phenotypes of individual CTCs collected from xenograft tumor-bearing mice and colorectal (CRC) patients at different tumor stages. We obtained a correlation between CTC characterization and clinical tumor stage and treatment response. The developed IMD offers a high-throughput, convenient, and rapid strategy to study individual CTCs toward minimally invasive cancer therapy prediction and disease monitoring and has the potential to be translated to clinic for liquid biopsy.
- Subjects :
- Animals
Antigens, CD genetics
Biomarkers, Tumor genetics
Cadherins genetics
Cell Count
Cell Line, Tumor
Cell Separation instrumentation
Cell Separation methods
Colorectal Neoplasms diagnosis
Colorectal Neoplasms genetics
Colorectal Neoplasms pathology
Epithelial Cell Adhesion Molecule genetics
Female
Fluorescein-5-isothiocyanate chemistry
Fluorescent Dyes chemistry
Glycoconjugates chemistry
Heterografts
Humans
Liquid Biopsy
Mice
Mice, Inbred BALB C
Neoplasm Staging
Neoplastic Cells, Circulating pathology
Antigens, CD blood
Biomarkers, Tumor blood
Cadherins blood
Colorectal Neoplasms blood
Epithelial Cell Adhesion Molecule blood
Lab-On-A-Chip Devices
Neoplastic Cells, Circulating metabolism
Single-Cell Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 91
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31373191
- Full Text :
- https://doi.org/10.1021/acs.analchem.9b01647