Direct Cyanine-dCTP Labeling of DNA for Microarrays.

Authors :: Rando O
Source :: Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2019 Aug 01; Vol. 2019 (8). Date of Electronic Publication: 2019 Aug 01.
Publication Year :: 2019
Abstract: Similar to direct RNA labeling, direct DNA labeling with Cy-dCTP is the simplest and fastest method for labeling DNA. This is a standard Klenow labeling protocol in which Cy-dCTP is incorporated during the labeling reaction. After stopping the reaction, labeled nucleotide is separated from unreacted Cy-dCTP, and Cy3- and Cy5-labeled materials are combined for hybridization. This protocol is suitable for many applications, including detection of copy-number variation, nucleosome mapping, and other location analysis (e.g., chromatin immunoprecipitation [ChIP]-chip).<br /> (© 2019 Cold Spring Harbor Laboratory Press.)