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Inhibition of Pre-mRNA Splicing Promotes Root Hair Development in Arabidopsis thaliana.
- Source :
-
Plant & cell physiology [Plant Cell Physiol] 2019 Sep 01; Vol. 60 (9), pp. 1974-1985. - Publication Year :
- 2019
-
Abstract
- Root hairs protruding from epidermal cells increase the surface area for water absorption and nutrient uptake. Various environmental factors including light, oxygen concentration, carbon dioxide concentration, calcium and mycorrhizal associations promote root hair formation in Arabidopsis thaliana. Light regulates the expression of a large number of genes at the transcriptional and post-transcriptional levels; however, there is little information linking the light response to root hair development. In this study, we describe a novel mutant, light-sensitive root-hair development 1 (lrh1), that displays enhanced root hair development in response to light. Hypocotyl and root elongation was inhibited in the lrh1 mutant, which had a late flowering phenotype. We identified the gene encoding the p14 protein, a putative component of the splicing factor 3b complex essential for pre-mRNA splicing, as being responsible for the lrh1 phenotype. Indeed, regulation of alternative splicing was affected in lrh1 mutants and treatment with a splicing inhibitor mimicked the lrh1 phenotype. Genome-wide alterations in pre-mRNA splicing patterns including differential splicing events of light signaling- and circadian clock-related genes were found in lrh1 as well as a difference in transcriptional regulation of multiple genes including upregulation of essential genes for root hair development. These results suggest that pre-mRNA splicing is the key mechanism regulating root hair development in response to light signals.<br /> (� The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Subjects :
- Arabidopsis growth & development
Circadian Clocks genetics
Hypocotyl genetics
Hypocotyl growth & development
Mutation
Phenotype
Plant Roots genetics
Plant Roots growth & development
RNA, Plant genetics
Signal Transduction
Alternative Splicing
Arabidopsis genetics
Gene Expression Regulation, Plant
RNA Precursors genetics
RNA Splicing
Subjects
Details
- Language :
- English
- ISSN :
- 1471-9053
- Volume :
- 60
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Plant & cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 31368506
- Full Text :
- https://doi.org/10.1093/pcp/pcz150