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Characterization of phenolic compounds from Eugenia supra-axillaris leaf extract using HPLC-PDA-MS/MS and its antioxidant, anti-inflammatory, antipyretic and pain killing activities in vivo.
- Source :
-
Scientific reports [Sci Rep] 2019 Jul 31; Vol. 9 (1), pp. 11122. Date of Electronic Publication: 2019 Jul 31. - Publication Year :
- 2019
-
Abstract
- Reactive oxygen species (ROS) are involved in the pathophysiology of several health disorders, among others inflammation. Polyphenols may modulate ROS related disorders. In this work, thirty-two phenolic compounds were tentatively identified in a leaf extract from Eugenia supra-axillaris Spring. ex Mart. using HPLC-MS/MS, five of which were also individually isolated and identified. The extract displayed a substantial in vitro antioxidant potential and was capable of decreasing ROS production and hsp-16.2 expression under oxidative stress conditions in vivo in the Caenorhabditis elegans model. Also, the extract showed higher inhibitory selectivity towards COX-2 than COX-1 in vitro with higher selectivity towards COX-2 than that of diclofenac. The extract also exhibited anti-inflammatory properties: It attenuated the edema thickness in a dose dependent fashion in carrageenan-induced hind-paw odema in rats. In addition, the extract reduced the carrageenan-induced leukocyte migration into the peritoneal cavity at the highest dose. Furthermore, the extract showed antipyretic and analgesic activities in a mouse model. Eugenia supra-axillaris appears to be a promising candidate in treating inflammation, pain and related oxidative stress diseases.
- Subjects :
- Animals
Chromatography, High Pressure Liquid methods
Edema drug therapy
Edema metabolism
Inflammation drug therapy
Male
Mice
Oxidative Stress drug effects
Pain drug therapy
Pain metabolism
Phenols chemistry
Rats
Rats, Wistar
Reactive Oxygen Species metabolism
Tandem Mass Spectrometry methods
Analgesics pharmacology
Anti-Inflammatory Agents pharmacology
Antioxidants physiology
Antipyretics pharmacology
Eugenia chemistry
Plant Extracts pharmacology
Plant Leaves chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31366955
- Full Text :
- https://doi.org/10.1038/s41598-019-46946-7