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Molecular Surveillance of Drug Resistance of Plasmodium falciparum Isolates Imported from Angola in Henan Province, China.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2019 Sep 23; Vol. 63 (10). Date of Electronic Publication: 2019 Sep 23 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Angola was the main origin country for the imported malaria in Henan Province, China. Antimalarial drug resistance has posed a threat to the control and elimination of malaria. Several molecular markers were confirmed to be associated with the antimalarial drug resistance, such as pfcrt , pfmdr1 , pfdhfr , pfdhps , and K13. This study evaluated the drug resistance of the 180 imported Plasmodium falciparum isolates from Angola via nested PCR using Sanger sequencing. The prevalences of pfcrt C <subscript>72</subscript> V <subscript>73</subscript> M <subscript>74</subscript> N <subscript>75</subscript> K <subscript>76</subscript> , pfmdr1 N <subscript>86</subscript> Y <subscript>184</subscript> S <subscript>1034</subscript> N <subscript>1042</subscript> D <subscript>1246</subscript> , pfdhfr A <subscript>16</subscript> N <subscript>51</subscript> C <subscript>59</subscript> S <subscript>108</subscript> D <subscript>139</subscript> I <subscript>164</subscript> , and pfdhps S <subscript>436</subscript> A <subscript>437</subscript> A <subscript>476</subscript> K <subscript>540</subscript> A <subscript>581</subscript> were 69.4%, 59.9%, 1.3% and 6.3%, respectively. Three nonsynonymous (A578S, M579I, and Q613E) and one synonymous (R471R) mutation of K13 were found, the prevalences of which were 2.5% and 1.3%, respectively. The single nucleotide polymorphisms (SNPs) in pfcrt , pfmdr1 , pfdhfr , and pfdhps were generally shown as multiple mutations. The mutant prevalence of pfcrt reduced gradually, but pfdhfr and pfdhps still showed high mutant prevalence, while pfmdr1 was relatively low. The mutation of the K13 gene was rare. Molecular surveillance of artemisinin (ART) resistance will be used as a tool to evaluate the real-time efficacy of the artemisinin-based combination therapies (ACTs) and the ART resistance situation.<br /> (Copyright © 2019 American Society for Microbiology.)
- Subjects :
- Amino Acid Substitution
Angola epidemiology
Antimalarials pharmacology
Artemisinins pharmacology
China epidemiology
Dihydropteroate Synthase metabolism
Epidemiological Monitoring
Gene Expression
Humans
Malaria, Falciparum drug therapy
Malaria, Falciparum epidemiology
Malaria, Falciparum parasitology
Membrane Transport Proteins metabolism
Molecular Epidemiology
Multidrug Resistance-Associated Proteins metabolism
Plasmodium falciparum drug effects
Plasmodium falciparum growth & development
Plasmodium falciparum metabolism
Protozoan Proteins metabolism
Tetrahydrofolate Dehydrogenase metabolism
Travel
Dihydropteroate Synthase genetics
Drug Resistance genetics
Membrane Transport Proteins genetics
Multidrug Resistance-Associated Proteins genetics
Plasmodium falciparum genetics
Polymorphism, Single Nucleotide
Protozoan Proteins genetics
Tetrahydrofolate Dehydrogenase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 63
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 31358591
- Full Text :
- https://doi.org/10.1128/AAC.00552-19