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Epitope Mapping of Anti-Diacylglycerol Kinase Zeta Monoclonal Antibody DzMab-1 for Immunohistochemical Analyses.
- Source :
-
Monoclonal antibodies in immunodiagnosis and immunotherapy [Monoclon Antib Immunodiagn Immunother] 2019 Aug; Vol. 38 (4), pp. 175-178. Date of Electronic Publication: 2019 Jul 29. - Publication Year :
- 2019
-
Abstract
- The diacylglycerol kinases (DGKs) catalyze the phosphorylation of the cell membrane lipid diacylglycerol (DG), which is important in lipid biochemistry and signal transduction into phosphatidic acid. DG-mediated signal transduction downstream of the T cell receptor has been reported to be terminated by DGKζ, 1 of 10 DGK isoforms in most cases. We previously established an anti-DGKζ monoclonal antibody (mAb) DzMab-1 (rat IgG <subscript>1</subscript> , kappa), which reacts with both mouse DGKζ and human DGKζ (hDGKζ). In this study, we characterized the binding epitope of DzMab-1 using Western blotting, and found that Met1 and Pro3 residues of hDGKζ are important for facilitating DzMab-1 binding to hDGKζ. Furthermore, DzMab-1 was shown to be useful for immunohistochemical analyses for formalin-fixed paraffin-embedded HeLa cells. These findings could be applied for the production of more functional anti-hDGKζ mAbs.
Details
- Language :
- English
- ISSN :
- 2167-9436
- Volume :
- 38
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Monoclonal antibodies in immunodiagnosis and immunotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 31355694
- Full Text :
- https://doi.org/10.1089/mab.2019.0020