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Dynamic nuclear polarization magnetic resonance imaging and the oxygen-sensitive paramagnetic agent OX63 provide a noninvasive quantitative evaluation of kidney hypoxia in diabetic mice.
- Source :
-
Kidney international [Kidney Int] 2019 Sep; Vol. 96 (3), pp. 787-792. Date of Electronic Publication: 2019 May 24. - Publication Year :
- 2019
-
Abstract
- Renal hypoxia may play an important role in the progression of diabetic nephropathy. However, tools that noninvasively and quantitatively measure oxygen tension in the kidney are lacking. Here, we evaluated the feasibility of a noninvasive and quantitative imaging technique using dynamic nuclear polarization magnetic resonance imaging (DNP-MRI) in combination with the oxygen-sensitive paramagnetic agent OX63 for measuring oxygen tension in the kidney. Our results demonstrate that the DNP-MRI technique can yield quantitative maps of oxygen tension in the mouse renal cortex. Using this procedure, we also showed that oxygen tension was less elevated in the renal cortex of both streptozotocin-induced type 1 diabetic mice and db/db mice, a model of type 2 diabetes, than in the renal cortex of age-matched control mice of each respective model. Oxygen tension in streptozotocin-exposed mice was significantly improved by insulin treatment. Thus, the noninvasive and quantitative DNP-MRI technique appears to be useful for studying the pathophysiological role of hypoxia.<br /> (Copyright © 2019 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Hypoxia
Diabetes Mellitus, Experimental chemically induced
Diabetes Mellitus, Type 1 chemically induced
Diabetic Nephropathies pathology
Disease Models, Animal
Humans
Indenes administration & dosage
Kidney Cortex pathology
Mice
Oxygen analysis
Streptozocin toxicity
Trityl Compounds administration & dosage
Diabetes Mellitus, Experimental complications
Diabetes Mellitus, Type 1 complications
Diabetic Nephropathies diagnosis
Kidney Cortex diagnostic imaging
Magnetic Resonance Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1523-1755
- Volume :
- 96
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Kidney international
- Publication Type :
- Academic Journal
- Accession number :
- 31345583
- Full Text :
- https://doi.org/10.1016/j.kint.2019.04.034