Back to Search
Start Over
Intracellular Imaging of Glutathione with MnO 2 Nanosheet@Ru(bpy) 3 2+ -UiO-66 Nanocomposites.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2019 Sep 04; Vol. 11 (35), pp. 31693-31699. Date of Electronic Publication: 2019 Aug 20. - Publication Year :
- 2019
-
Abstract
- Fluorescent detection of glutathione (GSH) in the living system has attracted much attention, but current fluorescent probes are usually exposed to the exterior environment, leading to photobleaching and premature leakage and subsequently limiting the sensitivity and photostability. Herein, luminescent metal-organic frameworks [Ru(bpy) <subscript>3</subscript> <superscript>2+</superscript> encapsulated in UiO-66] coated with manganese dioxide nanosheets [MnO <subscript>2</subscript> NS@Ru(bpy) <subscript>3</subscript> <superscript>2+</superscript> -UiO-66] were prepared by an in situ growth method and further explored to construct a GSH-switched fluorescent sensing platform. Because of the splendid fluorescence quenching ability, special probe leakage blocking role and distinguished recognition of the MnO <subscript>2</subscript> NS, and the improved fluorescence of Ru(bpy) <subscript>3</subscript> <superscript>2+</superscript> by UiO-66, a low background, highly sensitive and selective detection of GSH with a low limit of detection as 0.28 μM was realized. At the same time, the preparation of MnO <subscript>2</subscript> NS@Ru(bpy) <subscript>3</subscript> <superscript>2+</superscript> -UiO-66 nanocomposites is simple and less toxic, and there was no notable loss of cell survivability after being exposed to MnO <subscript>2</subscript> NS@Ru(bpy) <subscript>3</subscript> <superscript>2+</superscript> -UiO-66 below the concentrations of 120 μg mL <superscript>-1</superscript> for 24 h. Consequently, the results coming from this effort suggest that the new sensing platform will have a great potential in the detection of GSH in living cells.
- Subjects :
- HeLa Cells
Humans
Microscopy, Fluorescence
Rubidium chemistry
Rubidium pharmacology
Glutathione metabolism
Manganese Compounds chemistry
Manganese Compounds pharmacology
Metal-Organic Frameworks chemistry
Metal-Organic Frameworks pharmacology
Nanocomposites chemistry
Oxides chemistry
Oxides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 11
- Issue :
- 35
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 31339687
- Full Text :
- https://doi.org/10.1021/acsami.9b11025