Back to Search
Start Over
Variations in Oral Microbiota Composition Are Associated With a Risk of Throat Cancer.
- Source :
-
Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2019 Jul 03; Vol. 9, pp. 205. Date of Electronic Publication: 2019 Jul 03 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- In this study, a next-generation sequencing strategy on 16 S ribosomal RNA (16 S rRNA) gene was employed to analyze 70 oral samples from 32 patients with throat cancer, nine patients with vocal cord polyp, and 29 healthy individuals (normal controls). Using this strategy, we demonstrated, for the first time, that the salivary microbiota of cancer patients were significantly different from those of patients with a polyp and healthy individuals. We observed that the beta diversity of the cancer group was divergent from both the normal and polyp groups, while alpha-diversity indices such as the Chao1 estimator ( P = 8.1e-05), Simpson ( P = 0.0045), and Shannon ( P = 0.0071) were significantly reduced in cancer patients compared with patients containing a polyp and normal healthy individuals. Linear discriminant analysis (LDA) and Kruskal-Wallis test analyses and real-time quantitative polymerase chain reaction (qPCR) verification test revealed that the genera Aggregatibacter, Pseudomonas, Bacteroides , and Ruminiclostridium were significantly enriched in the throat cancer group compared with the vocal cord polyp and normal control groups (score value >2). Finally, diagnostic models based on putatively important constituent bacteria were constructed with 87.5% accuracy [area under the curve (AUC) = 0.875, 95% confidence interval (CI): 0.695-1]. In summary, in this study we characterized, for the first time, the oral microbiota of throat cancer patients without smoking history. We speculate that these results will help in the pathogenic mechanism and early diagnosis of throat cancer.
- Subjects :
- Bacteria genetics
DNA, Bacterial genetics
Early Diagnosis
Female
High-Throughput Nucleotide Sequencing
Humans
Male
Middle Aged
Oral Health
Pharyngeal Neoplasms diagnosis
Phylogeny
Polyps
RNA, Ribosomal, 16S genetics
Real-Time Polymerase Chain Reaction
Saliva microbiology
Sequence Analysis, DNA
Bacteria classification
Biodiversity
Microbiota genetics
Pharyngeal Neoplasms microbiology
Pharynx microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 2235-2988
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- Frontiers in cellular and infection microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31334130
- Full Text :
- https://doi.org/10.3389/fcimb.2019.00205