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Contrasting Gene Expression Profiles of Monocytes and Lymphocytes From Peste-Des-Petits-Ruminants Virus Infected Goats.
- Source :
-
Frontiers in immunology [Front Immunol] 2019 Jul 05; Vol. 10, pp. 1463. Date of Electronic Publication: 2019 Jul 05 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- In this study, transcriptome analysis of PPRV infected PBMC subsets-T helper cells, T cytotoxic cells, monocytes, and B lymphocytes was done to delineate their role in host response. PPRV was found to infect lymphocytes and not monocytes. The established receptor for PPRV-SLAM was found downregulated in lymphocytes and non-differentially expressed in monocytes. A profound deviation in the global gene expression profile with a large number of unique upregulated genes (851) and downregulated genes (605) was observed in monocytes in comparison to lymphocytes. ISGs-ISG15, Mx1, Mx2, RSAD2, IFIT3, and IFIT5 that play a role in antiviral response and the genes for viral sensors-MDA5, LGP2, and RIG1, were found to be upregulated in lymphocytes and downregulated in monocytes. The transcription factors-IRF-7 and STAT-1 that regulate expression of most of the ISGs were found activated in lymphocytes and not in monocytes. Interferon signaling pathway and RIG1 like receptor signaling pathway were found activated in lymphocytes and not in monocytes. This contrast in gene expression profiles and signaling pathways indicated the predominant role of lymphocytes in generating the antiviral response against PPRV in goats, thus, giving us new insights into host response to PPRV.
- Subjects :
- Animals
Gene Expression Profiling
Goat Diseases virology
Goats immunology
Host-Pathogen Interactions immunology
Peste-des-Petits-Ruminants immunology
Peste-des-Petits-Ruminants virology
Signaling Lymphocytic Activation Molecule Family Member 1 metabolism
B-Lymphocytes immunology
Goat Diseases immunology
Monocytes immunology
Peste-des-petits-ruminants virus immunology
T-Lymphocytes, Cytotoxic immunology
T-Lymphocytes, Helper-Inducer immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 31333643
- Full Text :
- https://doi.org/10.3389/fimmu.2019.01463