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Decreased In Vitro Artemisinin Sensitivity of Plasmodium falciparum across India.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2019 Sep 23; Vol. 63 (10). Date of Electronic Publication: 2019 Sep 23 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Artemisinin-based combination therapy (ACT) has been used to treat uncomplicated Plasmodium falciparum infections in India since 2004. Since 2008, a decrease in artemisinin effectiveness has been seen throughout the Greater Mekong Subregion. The geographic proximity and ecological similarities of northeastern India to Southeast Asia may differentially affect the long-term management and sustainability of ACT in India. In order to collect baseline data on variations in ACT sensitivity in Indian parasites, 12 P. falciparum isolates from northeast India and 10 isolates from southwest India were studied in vitro Ring-stage survival assay (RSA) showed reduced sensitivity to dihydroartemisinin in 50% of the samples collected in northeast India in 2014 and 2015. Two of the 10 assayed samples from the southwest region of India from as far back as 2012 also showed decreased sensitivity to artemisinin. In both these regions, kelch gene sequences were not predictive of reduced artemisinin sensitivity, as measured by RSA. The present data justify future investments in integrated approaches involving clinical follow-up studies, in vitro survival assays, and molecular markers for tracking potential changes in the effectiveness of artemisinin against P. falciparum throughout India.<br /> (Copyright © 2019 Chakrabarti et al.)
- Subjects :
- Antimalarials pharmacology
Base Sequence
Drug Resistance
Erythrocytes drug effects
Erythrocytes parasitology
Gene Expression
Geography
Humans
India epidemiology
Kelch Repeat
Life Cycle Stages genetics
Malaria, Falciparum drug therapy
Malaria, Falciparum parasitology
Mutation
Plasmodium falciparum genetics
Plasmodium falciparum growth & development
Plasmodium falciparum metabolism
Protozoan Proteins metabolism
Artemisinins pharmacology
Life Cycle Stages drug effects
Malaria, Falciparum epidemiology
Plasmodium falciparum drug effects
Protozoan Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 63
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 31332065
- Full Text :
- https://doi.org/10.1128/AAC.00101-19