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Caveolin-1 Endows Order in Cholesterol-Rich Detergent Resistant Membranes.
- Source :
-
Biomolecules [Biomolecules] 2019 Jul 17; Vol. 9 (7). Date of Electronic Publication: 2019 Jul 17. - Publication Year :
- 2019
-
Abstract
- Cholesterol-enriched functional portions of plasma membranes, such as caveolae and rafts, were isolated from lungs of wild-type (WT) and caveolin-1 knockout (Cav-1 KO) mice within detergent resistant membranes (DRMs). To gain insight into their molecular composition we performed proteomic and lipid analysis on WT and Cav-1 KO-DRMs that showed predicted variations of proteomic profiles and negligible differences in lipid composition, while Langmuir monolayer technique and small and wide-angle X-ray scattering (SAXS-WAXS) were here originally introduced to study DRMs biophysical association state. Langmuir analysis of Cav-1 containing DRMs displayed an isotherm with a clear-cut feature, suggesting the coexistence of the liquid-ordered ( L <subscript>o</subscript> ) phase typical of the raft structure, namely "cholesterol-rich L <subscript>o</subscript> phase," with a phase fully missing in Cav-1 KO that we named "caveolin-induced L <subscript>o</subscript> phase." Furthermore, while the sole lipid component of both WT and KO-DRMs showed qualitatively similar isotherm configuration, the reinsertion of recombinant Cav-1 into WT-DRMs lipids restored the WT-DRM pattern. X-ray diffraction results confirmed that Cav-1 causes the formation of a "caveolin-induced L <subscript>o</subscript> phase," as suggested by Langmuir experiments, allowing us to speculate about a possible structural model. These results show that the unique molecular link between Cav-1 and cholesterol can spur functional order in a lipid bilayer strictly derived from biological sources.<br />Competing Interests: The authors declare no conflicts of interest.
Details
- Language :
- English
- ISSN :
- 2218-273X
- Volume :
- 9
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biomolecules
- Publication Type :
- Academic Journal
- Accession number :
- 31319608
- Full Text :
- https://doi.org/10.3390/biom9070287