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Antioxidant potentials and effects on the hematology and osmotic fragility scores of a polyherbal formulation used in Southeast Nigeria.
- Source :
-
Journal of basic and clinical physiology and pharmacology [J Basic Clin Physiol Pharmacol] 2019 Jul 17; Vol. 30 (4). Date of Electronic Publication: 2019 Jul 17. - Publication Year :
- 2019
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Abstract
- Background In this study, the hematological and antioxidant potential as well as the osmotic fragility effects of a Nigerian polyherbal formulation were evaluated. Materials and methods A total of 40 fats were divided into four groups of 10 rats each. Group 1 served as the control group, and the rest were assigned increasing daily oral administration of the extract for 28 days. At the end of treatment, blood was collected for hematological and osmotic fragility studies. The free radical scavenging effect of the extract was investigated via different in vitro models as well. Results Results showed that the nitric oxide scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) activities of the extract were significant (p < 0.05) and compared favorably with that of vitamin C. At 200 and 400 μg/mL, the nitric oxide scavenging activities for Ajumbise Polyherbal Extract (APE) were 60.71 ± 0.25% and 59.49 ± 0.98%, respectively, whereas for the same concentrations of vitamin C, 74.60 ± 0.25% and 85.24 ± 0.14 scavenging activities were obtained. The (DPPH) activity at 100 μg/mL was 81.24 ± 0.02% for the extract and 96.22 ± 0.18% for vitamin C. However, at all concentrations, the extract had significantly lower Ferric Reducing Antioxidant Power (FRAP) activity than vitamin C. Red blood cell counts (RBCC), hemoglobin and packed cell volume values (PCV) were significantly lowered only in groups treated with 400 and 800 mg/kg of the extract (p < 0.05), whereas other RBCC parameters and white blood cell counts (WBCC) were not significantly affected (p < 0.05). Platelet (PLT) count was also significantly lowered in all extract-treated groups. The extract also significantly reduced RBCC percentage hemolysis (p < 0.05). Conclusions Ajumbise polyherbal may be free of hematoxicity and may improve the integrity of the RBC membrane due to its appreciable antioxidant activity.
- Subjects :
- Animals
Ascorbic Acid pharmacology
Biphenyl Compounds pharmacology
Blood Platelets drug effects
Erythrocytes drug effects
Flavonoids pharmacology
Hematology methods
Hemolysis drug effects
Leukocytes drug effects
Nigeria
Nitric Oxide metabolism
Phenols pharmacology
Phytotherapy methods
Picrates pharmacology
Rats
Antioxidants pharmacology
Osmotic Fragility drug effects
Plant Extracts pharmacology
Plants, Medicinal chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2191-0286
- Volume :
- 30
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of basic and clinical physiology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 31314739
- Full Text :
- https://doi.org/10.1515/jbcpp-2017-0099