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Resident muscle stem cells are not required for testosterone-induced skeletal muscle hypertrophy.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2019 Oct 01; Vol. 317 (4), pp. C719-C724. Date of Electronic Publication: 2019 Jul 17. - Publication Year :
- 2019
-
Abstract
- It is postulated that testosterone-induced skeletal muscle hypertrophy is driven by myonuclear accretion as the result of satellite cell fusion. To directly test this hypothesis, we utilized the Pax7-DTA mouse model to deplete satellite cells in skeletal muscle followed by testosterone administration. Pax7-DTA mice (6 mo of age) were treated for 5 days with either vehicle [satellite cell replete (SC+)] or tamoxifen [satellite cell depleted (SC-)]. Following a washout period, a testosterone propionate or sham pellet was implanted for 21 days. Testosterone administration caused a significant increase in muscle fiber cross-sectional area in SC+ and SC- mice in both oxidative (soleus) and glycolytic (plantaris and extensor digitorum longus) muscles. In SC+ mice treated with testosterone, there was a significant increase in both satellite cell abundance and myonuclei that was completely absent in testosterone-treated SC- mice. These findings provide direct evidence that testosterone-induced muscle fiber hypertrophy does not require an increase in satellite cell abundance or myonuclear accretion.Listen to a podcast about this Rapid Report with senior author E. E. Dupont-Versteegden (https://ajpcell.podbean.com/e/podcast-on-paper-that-shows-testosterone-induced-skeletal-muscle-hypertrophy-does-not-need-muscle-stem-cells/).
- Subjects :
- Animals
Disease Models, Animal
Hypertrophy chemically induced
Mice, Transgenic
Muscle Fibers, Skeletal physiology
PAX7 Transcription Factor genetics
Satellite Cells, Skeletal Muscle physiology
Muscle Fibers, Skeletal drug effects
Satellite Cells, Skeletal Muscle drug effects
Stem Cells drug effects
Testosterone pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1563
- Volume :
- 317
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 31314585
- Full Text :
- https://doi.org/10.1152/ajpcell.00260.2019