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Frequency and expression of genes involved in adhesion and biofilm formation in Staphylococcus aureus strains isolated from periodontal lesions.
- Source :
-
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi [J Microbiol Immunol Infect] 2021 Apr; Vol. 54 (2), pp. 267-275. Date of Electronic Publication: 2019 Jul 03. - Publication Year :
- 2021
-
Abstract
- Background/purpose: The aim of this study was to characterize the Staphylococcus aureus strains isolated from periodontal lesions of patients, to determine the expression of genes involved in cell adhesion upon their infection of human epithelial cells using an in vitro model, its biofilm formation, and its resistance to antibiotics.<br />Methods: S. aureus was analysed by PCR, Kirby-Bauer, and pulsed-field gel electrophoresis (PFGE), measuring gene expression by real-time PCR after infection of human cells in vitro.<br />Results: S. aureus was identified in 18.6% (50/268) of the samples. All strains (n = 50) possessed the virulence genes spa (Staphylococcal protein A), coa (coagulase), and icaAB (intercellular adhesin); 96% (n = 48) possessed clfB (clumping factor B), and 88% (n = 44) possessed ebps (elastin-binding protein) and sdrD (serine aspartate repeat protein D). All strains were resistant to methicillin, ampicillin, dicloxacillin, cefotaxime, and penicillin, and were multidrug resistant to 6-12 antibiotics. PFGE analysis showed 37 different pulsed-field types and most strains (60.4%) had a unique pulsed-field type. Twenty-four distinct combinations of virulence genes and antibiotic-resistant phenotypes were identified.<br />Conclusion: Although S. aureus has been considered a transient member of the oral microbiota, our results indicate a high-level expression of virulence genes and multidrug resistance in the strains isolated from periodontal lesions. These strains might complicate the successful treatment of the disease.<br /> (Copyright © 2019. Published by Elsevier B.V.)
- Subjects :
- Adhesins, Bacterial drug effects
Anti-Bacterial Agents pharmacology
Antigens, Bacterial genetics
Biofilms drug effects
Cell Line
Disk Diffusion Antimicrobial Tests
Drug Resistance, Multiple, Bacterial genetics
Electrophoresis, Gel, Pulsed-Field
Epithelial Cells
Female
Gene Expression Regulation, Bacterial
Genotype
Humans
Male
Mexico
Microbial Sensitivity Tests
Microbiota
Mouth microbiology
Phenotype
Polymerase Chain Reaction
Staphylococcal Infections microbiology
Staphylococcus aureus drug effects
Virulence genetics
Adhesins, Bacterial genetics
Biofilms growth & development
Staphylococcus aureus genetics
Staphylococcus aureus isolation & purification
Staphylococcus aureus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1995-9133
- Volume :
- 54
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 31300301
- Full Text :
- https://doi.org/10.1016/j.jmii.2019.05.010