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First report of megalocytivirus (iridoviridae) in cultured bluegill sunfish, Lepomis macrochirus, in China.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2019 Oct; Vol. 135, pp. 103617. Date of Electronic Publication: 2019 Jul 05. - Publication Year :
- 2019
-
Abstract
- The bluegill sunfish, Lepomis macrochirus, is an important aquacultural and recreational species in southern China because of its excellent taste, rapid growth rate, and good looks. At present, few pathogens are known to affect the bluegill sunfish. However, an iridovirus-like disease recently caused heavy losses to the bluegill sunfish aquaculture industry in Guangdong, China. We report that a virus, designated BSMIV-SD-20171020, was isolated from diseased bluegill sunfish in China. The isolate was efficiently propagated in a Chinese perch brain (CPB) cell line. The cytopathic effect was observed, the MCP gene PCR amplified, and the virus observed with electron microscopy. Its viral titer in CPB cells reached 10 <superscript>4.13</superscript> TCID <subscript>50</subscript> mL <superscript>-1</superscript> . The mortality rate was 100% when bluegill sunfish were challenged with BSMIV-SD-20171020 at a dose of 10 <superscript>3.13</superscript> TCID <subscript>50</subscript> /fish. A histopathological examination revealed basophilic hypertrophied cells in the intestine, liver, and spleen. A nucleotide sequence alignment and phylogenetic analysis of the major capsid protein revealed that isolate BSMIV-SD-20171020 is the species Infectious spleen and kidney necrosis virus (ISKNV), in the genus Megalocytivirus.<br /> (Copyright © 2019. Published by Elsevier Ltd.)
- Subjects :
- Animals
Aquaculture
Brain
Capsid Proteins classification
Capsid Proteins genetics
Cell Line
China
DNA Virus Infections pathology
Fish Diseases pathology
Fishes
Iridoviridae genetics
Iridoviridae pathogenicity
Kidney pathology
Kidney virology
Liver pathology
Liver virology
Perches
Phylogeny
Sequence Analysis, DNA veterinary
Spleen pathology
Spleen virology
DNA Virus Infections veterinary
DNA Virus Infections virology
Fish Diseases virology
Iridoviridae classification
Iridoviridae isolation & purification
Perciformes virology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 135
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 31283962
- Full Text :
- https://doi.org/10.1016/j.micpath.2019.103617