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Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site.
- Source :
-
BMC complementary and alternative medicine [BMC Complement Altern Med] 2019 Jul 06; Vol. 19 (1), pp. 163. Date of Electronic Publication: 2019 Jul 06. - Publication Year :
- 2019
-
Abstract
- Background: Therapeutic vaccines against cervical cancer remain ineffective. Previously, we demonstrated that blocking the signalling of a cytokine, interleukin 10, at the time of immunisation elicited significantly higher numbers of antigen specific T cells and inhibited tumour growth in mice.<br />Results: In the current paper, we demonstrate, in a HPV16 E6/E7 transformed TC-1 tumour mouse model, that despite increased antigen specific T cell numbers, blocking IL-10 signalling at the time of immunisation does not increase the survival time of the TC-1 tumour bearing mice compared to mice receiving the same immunisation with no IL-10 signalling blockade. Moreover, the function of tumour infiltrating T cells isolated 3 weeks post TC-1 transplantation is more suppressed than those isolated 2 weeks after tumour inoculation. We demonstrate that synthesized caerin peptides, derived from amphibian skin secretions, 1) were able to inhibit TC-1 tumour growth both in vitro and in vivo; 2) are environmentally stable; and 3) promote the secretion of pro-inflammatory interlukine-6 by TC-1 cells. Notably caerin peptides were able to increase the survival time of TC-1 tumour bearing mice after therapeutic vaccination with a HPV16E7 peptide-based vaccine containing IL-10 inhibitor, via recruiting increased levels of T cells to the tumour site.<br />Conclusion: Caerin peptides increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site.
- Subjects :
- Amphibian Proteins therapeutic use
Animals
Antimicrobial Cationic Peptides therapeutic use
Cancer Vaccines pharmacology
Drug Screening Assays, Antitumor
Female
HeLa Cells
Humans
Interleukin-10 antagonists & inhibitors
Interleukin-6 metabolism
Lymphocytes, Tumor-Infiltrating metabolism
Mice, Inbred C57BL
Neoplasm Transplantation
Neoplasms, Experimental drug therapy
T-Lymphocytes metabolism
Amphibian Proteins pharmacology
Antimicrobial Cationic Peptides pharmacology
Cancer Vaccines therapeutic use
Lymphocytes, Tumor-Infiltrating drug effects
T-Lymphocytes drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6882
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC complementary and alternative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 31277636
- Full Text :
- https://doi.org/10.1186/s12906-019-2571-z