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Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli.

Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli.

Authors :
Crawford IP
Wilde A
Yelverton EM
Figurski D
Hedges RW
Source :
Molecular biology and evolution [Mol Biol Evol] 1986 Sep; Vol. 3 (5), pp. 449-58.
Publication Year :
1986

Abstract

The genes for the large and small subunits of anthranilate synthase (trpE and trpG, respectively) have been cloned from Pseudomonas aeruginosa PAC174 into E. coli by R-prime formation with the broad-host-range plasmid R68.44. Sequential subcloning into plasmid vectors reduced the active Pseudomonas DNA fragment to a length of 3.1 kb. We obtained evidence that this region contains the promoter for its own expression and retains a vestigial regulatory response to tryptophan scarcity or excess.

Details

Language :
English
ISSN :
0737-4038
Volume :
3
Issue :
5
Database :
MEDLINE
Journal :
Molecular biology and evolution
Publication Type :
Academic Journal
Accession number :
3127655
Full Text :
https://doi.org/10.1093/oxfordjournals.molbev.a040409