Back to Search
Start Over
Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli.
Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli.
- Source :
-
Molecular biology and evolution [Mol Biol Evol] 1986 Sep; Vol. 3 (5), pp. 449-58. - Publication Year :
- 1986
-
Abstract
- The genes for the large and small subunits of anthranilate synthase (trpE and trpG, respectively) have been cloned from Pseudomonas aeruginosa PAC174 into E. coli by R-prime formation with the broad-host-range plasmid R68.44. Sequential subcloning into plasmid vectors reduced the active Pseudomonas DNA fragment to a length of 3.1 kb. We obtained evidence that this region contains the promoter for its own expression and retains a vestigial regulatory response to tryptophan scarcity or excess.
Details
- Language :
- English
- ISSN :
- 0737-4038
- Volume :
- 3
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular biology and evolution
- Publication Type :
- Academic Journal
- Accession number :
- 3127655
- Full Text :
- https://doi.org/10.1093/oxfordjournals.molbev.a040409