Reclassification of ' Polyangium brachysporum ' DSM 7029 as Schlegelella brevitalea sp. nov.

Strain DSM 7029, isolated from a soil sample in Greece, can produce antitumour glidobactins, and has been found, as a heterologous host, to produce useful nonribosomal peptide synthetase-polyketide synthase hybrid molecules known as epothilones. This strain was originally named ' Polyangium brachysporum ' of the family Polyangiaceae and the order Myxococcales . However, phylogenetic analysis of the 16S rRNA gene sequence of strain DSM 7029 indicated that it was clustered with members of Schlegelella . Significant growth occurred at 25-42 °C, pH 5.0-10.0 and in the presence of 0-0.2 % (w/v) NaCl. The predominant ubiquinone was Q-8. The major fatty acids were C _16 : 1 ω7 c /C _16 : 1 ω6 c , C _16 : 0 and C _18 : 1 ω7 c . The G+C content of genomic DNA was 67.51 mol%. The strain was clearly distinguishable from other neighbouring Schlegelella members and genera Caldimonas and Zhizhongheella , using phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics and genome analysis. Therefore, strain DSM 7029 represents a novel species of the genus Schlegelella , for which the name Schlegelella brevitalea sp. nov. is proposed.