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PRDM14 and BLIMP1 control the development of chicken primordial germ cells.
- Source :
-
Developmental biology [Dev Biol] 2019 Nov 01; Vol. 455 (1), pp. 32-41. Date of Electronic Publication: 2019 Jul 02. - Publication Year :
- 2019
-
Abstract
- The differentiation of primordial germ cells (PGCs) is a fundamental step in development. PR domain-containing protein 14 (PRDM14) and B lymphocyte-induced maturation protein 1 (BLIMP1) play pivotal roles in mouse PGC specification. In the present study, we assessed the roles of chicken orthologs of PRDM14 and BLIMP1 in PGC development. PRDM14 and BLIMP1 were expressed in blastodermal cells and PGCs. The in vivo knockdown of PRDM14 or BLIMP1 by introducing a replication-competent retroviral vector expressing shRNAs to the blastodermal stage of embryos reduced the number of SSEA-1 or chicken vasa homologue-positive PGCs on day 5.5-6.5. Since the inhibition of Activin receptor-like kinase 4/5/7 in cultured PGCs reduced the expression of PRDM14, BLIMP1, and NANOG, and that of MEK inhibited PRDM14 expression, the expression of these genes seems to be controlled by Activin A and FGF2 signaling. Overall, PRDM14, BLIMP1, and NANOG seem to be involved in the self-renewal of PGCs in cultured PGCs and embryos.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Avian Proteins metabolism
Blastoderm cytology
Blastoderm metabolism
Cell Self Renewal genetics
Cells, Cultured
Chick Embryo
Chickens
Germ Cells cytology
Lewis X Antigen genetics
Lewis X Antigen metabolism
Nanog Homeobox Protein genetics
Nanog Homeobox Protein metabolism
Positive Regulatory Domain I-Binding Factor 1 metabolism
RNA Interference
Avian Proteins genetics
Cell Differentiation genetics
Gene Expression Regulation, Developmental
Germ Cells metabolism
Positive Regulatory Domain I-Binding Factor 1 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-564X
- Volume :
- 455
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 31271752
- Full Text :
- https://doi.org/10.1016/j.ydbio.2019.06.018