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Forkhead box O3 protects the heart against paraquat-induced aging-associated phenotypes by upregulating the expression of antioxidant enzymes.
- Source :
-
Aging cell [Aging Cell] 2019 Oct; Vol. 18 (5), pp. e12990. Date of Electronic Publication: 2019 Jul 01. - Publication Year :
- 2019
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Abstract
- Paraquat (PQ) promotes cell senescence in brain tissue, which contributes to Parkinson's disease. Furthermore, PQ induces heart failure and oxidative damage, but it remains unknown whether and how PQ induces cardiac aging. Here, we demonstrate that PQ induces phenotypes associated with senescence of cardiomyocyte cell lines and results in cardiac aging-associated phenotypes including cardiac remodeling and dysfunction in vivo. Moreover, PQ inhibits the activation of Forkhead box O3 (FoxO3), an important longevity factor, both in vitro and in vivo. We found that PQ-induced senescence phenotypes, including proliferation inhibition, apoptosis, senescence-associated β-galactosidase activity, and p16 <superscript>INK4a</superscript> expression, were significantly enhanced by FoxO3 deficiency in cardiomyocytes. Notably, PQ-induced cardiac remolding, apoptosis, oxidative damage, and p16 <superscript>INK4a</superscript> expression in hearts were exacerbated by FoxO3 deficiency. In addition, both in vitro deficiency and in vivo deficiency of FoxO3 greatly suppressed the activation of antioxidant enzymes including catalase (CAT) and superoxide dismutase 2 (SOD2) in the presence of PQ, which was accompanied by attenuation in cardiac function. The direct in vivo binding of FoxO3 to the promoters of the Cat and Sod2 genes in the heart was verified by chromatin immunoprecipitation (ChIP). Functionally, overexpression of Cat or Sod2 alleviated the PQ-induced senescence phenotypes in FoxO3-deficient cardiomyocyte cell lines. Overexpression of FoxO3 and CAT in hearts greatly suppressed the PQ-induced heart injury and phenotypes associated with aging. Collectively, these results suggest that FoxO3 protects the heart against an aging-associated decline in cardiac function in mice exposed to PQ, at least in part by upregulating the expression of antioxidant enzymes and suppressing oxidative stress.<br /> (© 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.)
- Subjects :
- Aging drug effects
Animals
Catalase genetics
Catalase metabolism
Heart drug effects
Mice
Mice, Knockout
Paraquat pharmacology
Phenotype
Superoxide Dismutase genetics
Superoxide Dismutase metabolism
Aging metabolism
Antioxidants metabolism
Forkhead Box Protein O3 metabolism
Paraquat antagonists & inhibitors
Protective Agents metabolism
Up-Regulation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1474-9726
- Volume :
- 18
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Aging cell
- Publication Type :
- Academic Journal
- Accession number :
- 31264342
- Full Text :
- https://doi.org/10.1111/acel.12990