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Identification of Potential Cerebrospinal Fluid Biomarkers To Discriminate between Infection and Sterile Inflammation in a Rat Model of Staphylococcus epidermidis Catheter Infection.
- Source :
-
Infection and immunity [Infect Immun] 2019 Aug 21; Vol. 87 (9). Date of Electronic Publication: 2019 Aug 21 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Staphylococcus epidermidis cerebrospinal fluid (CSF) shunt infection is a common complication of hydrocephalus treatment, creating grave neurological consequences for patients, especially when diagnosis is delayed. The current method of diagnosis relies on microbiological culture; however, awaiting culture results may cause treatment delays, or culture may fail to identify infection altogether, so newer methods are needed. To investigate potential CSF biomarkers of S. epidermidis shunt infection, we developed a rat model allowing for serial CSF sampling. We found elevated levels of interleukin-10 (IL-10), IL-1β, chemokine ligand 2 (CCL2), and CCL3 in the CSF of animals implanted with S. epidermidis -infected catheters compared to sterile controls at day 1 postinfection. Along with increased chemokine and cytokine expression early in infection, neutrophil influx was significantly increased in the CSF of animals with infected catheters, suggesting that coupling leukocyte counts with inflammatory mediators may differentiate infection from sterile inflammation. Mass spectrometry analysis revealed that the CSF proteome in sterile animals was similar to that in infected animals at day 1; however, by day 5 postinfection, there was an increase in the number of differently expressed proteins in the CSF of infected compared to sterile groups. The expansion of the proteome at day 5 postinfection was interesting, as bacterial burdens began to decline by this point, yet the CSF proteome data indicated that the host response remained active, especially with regard to the complement cascade. Collectively, these results provide potential biomarkers to distinguish S. epidermidis infection from sterile postoperative inflammation.<br /> (Copyright © 2019 Skar et al.)
- Subjects :
- Animals
Biomarkers cerebrospinal fluid
Catheter-Related Infections microbiology
Chemokines cerebrospinal fluid
Cytokines cerebrospinal fluid
Disease Models, Animal
Inflammation cerebrospinal fluid
Neutrophils cytology
Rats
Staphylococcal Infections microbiology
Catheter-Related Infections cerebrospinal fluid
Staphylococcal Infections cerebrospinal fluid
Staphylococcus epidermidis isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 87
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 31262978
- Full Text :
- https://doi.org/10.1128/IAI.00311-19