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Electroporation.

Authors :
Kumar P
Nagarajan A
Uchil PD
Source :
Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2019 Jul 01; Vol. 2019 (7). Date of Electronic Publication: 2019 Jul 01.
Publication Year :
2019

Abstract

Electroporation is a process in which brief electrical pulses create transient pores in the plasma membrane that allow nucleic acids to enter the cellular cytoplasm. Here, we provide information on the history, mechanism, and optimization of electroporation. We also describe nucleofection, an improvement of the electroporation technology that permits the introduction of nucleic acids directly into the nucleus.<br /> (© 2019 Cold Spring Harbor Laboratory Press.)

Details

Language :
English
ISSN :
1559-6095
Volume :
2019
Issue :
7
Database :
MEDLINE
Journal :
Cold Spring Harbor protocols
Publication Type :
Academic Journal
Accession number :
31262965
Full Text :
https://doi.org/10.1101/pdb.top096271