Back to Search
Start Over
Aerosol immunization by alginate coated mycobacterium (BCG/MIP) particles provide enhanced immune response and protective efficacy than aerosol of plain mycobacterium against M.tb. H37Rv infection in mice.
- Source :
-
BMC infectious diseases [BMC Infect Dis] 2019 Jul 01; Vol. 19 (1), pp. 568. Date of Electronic Publication: 2019 Jul 01. - Publication Year :
- 2019
-
Abstract
- Background: With the aim of preparing a more effective, safe and economical vaccine for tuberculosis, inhalable live mycobacterium formulations were evaluated.<br />Methods: Alginate particles in the size range of 2-4 μm were prepared by encapsulating live Bacille Calmette-Guérin (BCG) and "Mycobacterium indicus pranii" (MIP). These particles were characterized for their size, stability and release profile. Mice were immunized with liquid aerosol or dry powder aerosol (DPA) alginate encapsulated mycobacterium particles and their in-vitro recall response and infection with mycobacterium H37Rv were investigated.<br />Results: It was found that the DPA of alginate encapsulated mycobacterium particles invoked superior immune response and provided higher protection in mice than the liquid aerosol. The BCG encapsulated in alginate particles (BEAP) and MIP encapsulated in alginate particles (MEAP) were engulfed by bone marrow dendritic cells (BMDCs) and co-localized with lysosome. The MEAP/BEAP activated BMDCs exhibited higher chemotaxis movement and had enhanced ability of antigen presentation to T cells. The in-vitro recall response of BEAP/MEAP immunized mice when compared in terms of proliferation index and Interferon gamma (IFN-gamma) released by splenocytes and mediastinal lymph node cells was found to be higher than mice immunized by liquid aerosol of BCG/MIP. Finally, different groups of immunized mice were infected with M. tb H37Rv and after 16 weeks the Colony forming units (CFUs) in lung and spleen estimated. The bacilli burden in the BEAP/MEAP immunized mice was significantly less than the respective liquid aerosol immunized mice and the histopathology of BEAP/MEAP immunized mice lungs showed very little damage.<br />Conclusions: These inhale-able vaccines formulation of alginate coated live mycobacterium are more immunogenic as compared to the aerosol of bacilli and they provide better protection in mice when infected with H37Rv.
- Subjects :
- Alginates chemistry
Animals
BCG Vaccine immunology
Drug Delivery Systems methods
Interferon-gamma immunology
Lung drug effects
Lung microbiology
Mice, Inbred BALB C
Mice, Inbred C57BL
Mycobacterium avium Complex chemistry
Mycobacterium avium Complex immunology
Mycobacterium bovis chemistry
Mycobacterium bovis immunology
Mycobacterium tuberculosis immunology
Mycobacterium tuberculosis pathogenicity
Spleen microbiology
T-Lymphocytes immunology
T-Lymphocytes microbiology
Tuberculosis immunology
Tuberculosis Vaccines administration & dosage
Tuberculosis Vaccines immunology
Vaccination methods
Aerosols administration & dosage
Lung immunology
Tuberculosis prevention & control
Tuberculosis Vaccines pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2334
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 31262260
- Full Text :
- https://doi.org/10.1186/s12879-019-4157-2