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SEROLOGICAL DIAGNOSIS OF BAYLISASCARIS PROCYONIS IN PRIMATES USING A HUMAN ELISA TEST.
- Source :
-
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians [J Zoo Wildl Med] 2019 Jun 13; Vol. 50 (2), pp. 414-420. Date of Electronic Publication: 2019 Jun 13. - Publication Year :
- 2019
-
Abstract
- The usefulness of a human enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of Baylisascaris procyonis larva migrans was assessed in nonhuman primates (NHP). The test was originally developed as an assay performed on human samples at Purdue University. Six participating zoos submitted 258 NHP serum samples, spanning these major phylogenetic groups: 1) great apes ( n = 84), 2) lesser apes ( n = 17), 3) Old World monkeys ( n = 84), 4) New World monkeys ( n = 20), and 5) prosimians ( n = 53). Sera were tested in duplicate using a microtiter-well ELISA with B. procyonis larval excretory-secretory proteins as antigen, and serum from an experimentally infected baboon ( Papio anubis ) served as positive control. The ELISA clearly identified seropositive animals in all zoos. With putative cutoffs of optical density (OD) measured at 405 nm (OD405) of <0.150 = negative, 0.150-0.250 = indeterminate, and >0.250 = positive, 149 of 258 (57.8%) were clearly negative (mean OD 0.046), and 78 of 258 (30.2%) were clearly positive (mean OD 0.657, range 0.253-1.773), the rest being indeterminate. Of these, 15 were high positive with OD 1.095-1.773 (mean 1.314). Positive animals were seen from all zoos; 76 (97.4%) were great apes, lesser apes, or Old World monkeys. The four highest ODs were in a siamang ( Symphalangus syndactylus ), lion-tailed macaque ( Macaca silenus ), Sumatran orangutan ( Pongo abelii ), and western lowland gorilla ( Gorilla gorilla gorilla ), all from different zoos. Prosimians had a mean OD of 0.039 and New World monkeys 0.021, indicating that human reagents either did not work for these groups or few infected animals were represented. These results indicate that the human ELISA for B. procyonis works well for at least higher phylogeny NHP and that serologic evidence of infection is surprisingly common, correlating with what is known for exposure to this parasite in zoos.<br /> (Copyright 2019 by American Association of Zoo Veterinarians.)
- Subjects :
- Aging
Animals
Ascaridida Infections diagnosis
Humans
Primate Diseases blood
Primate Diseases diagnosis
Primates parasitology
Seroepidemiologic Studies
Serologic Tests
Species Specificity
Ascaridida Infections veterinary
Ascaridoidea isolation & purification
Enzyme-Linked Immunosorbent Assay methods
Primate Diseases parasitology
Primates blood
Subjects
Details
- Language :
- English
- ISSN :
- 1042-7260
- Volume :
- 50
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians
- Publication Type :
- Academic Journal
- Accession number :
- 31260208
- Full Text :
- https://doi.org/10.1638/2017-0207