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The Ultrashort Peptide OW: A New Antibiotic Adjuvant.
- Source :
-
Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2019; Vol. 20 (9), pp. 745-754. - Publication Year :
- 2019
-
Abstract
- Background: The over use of current antibiotics and low discovery rate of the new ones are leading to rapid development of multidrug-resistant pathogens worldwide. Antimicrobial peptides have shown promising results against multidrug-resistant bacteria.<br />Objective: To investigate the antimicrobial activity of a new ultrashort hexapeptide (OW).<br />Methods: The OW hexapeptide was designed and tested against different strains of bacteria with different levels of sensitivity. Bacterial susceptibility assays were performed according to the guidelines of the Clinical and Laboratory Institute (CLSI). The synergistic studies were then conducted using the Checkerboard assay. This was followed by checking the hemolytic effect of the hexapeptide against human blood cells and Human Embryonic Kidney cell line (HEK293). Finally, the antibiofilm activities of the hexapeptide were studied using the Biofilm Calgary method.<br />Results: Synergistic assays showed that OW has synergistic effects with antibiotics of different mechanisms of action. It showed an outstanding synergism with Rifampicin against methicillin resistant Staphylococcus aureus; ΣFIC value was 0.37, and the MIC value of Rifampicin was decreased by 85%. OW peptide also displayed an excellent synergism with Ampicillin against multidrug-resistant Pseudomonas aeruginosa, with ΣFIC value of less than 0.38 and a reduction of more than 96% in the MIC value of Ampicillin.<br />Conclusion: This study introduced a new ultrashort peptide (OW) with promising antimicrobial potential in the management of drug-resistant infectious diseases as a single agent or in combination with commonly used antibiotics. Further studies are needed to investigate the exact mechanism of action of these peptides.<br /> (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Subjects :
- Adjuvants, Pharmaceutic chemistry
Anti-Bacterial Agents chemistry
Biofilms drug effects
Drug Resistance, Multiple, Bacterial drug effects
Drug Synergism
HEK293 Cells
Humans
Microbial Sensitivity Tests
Oligopeptides chemistry
Pseudomonas aeruginosa drug effects
Adjuvants, Pharmaceutic pharmacology
Anti-Bacterial Agents pharmacology
Methicillin-Resistant Staphylococcus aureus drug effects
Oligopeptides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4316
- Volume :
- 20
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Current pharmaceutical biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 31258076
- Full Text :
- https://doi.org/10.2174/1389201020666190618111252