Achieving deep-level nutrient removal via combined denitrifying phosphorus removal and simultaneous partial nitrification-endogenous denitrification process in a single-sludge sequencing batch reactor.

The feasibility of coupling denitrifying phosphorus removal (DPR) with simultaneous partial nitrification-endogenous denitrification (SPNED) was investigated in a single-sludge sequencing batch reactor for deep-level nutrient removal from municipal and nitrate wastewaters. After 160-day operation, the DPR process simultaneously reduced most PO ₄ ^3- -P and NO ₃ ^- -N anoxically, and the SPNED process achieved further total nitrogen (TN) removal at low dissolved oxygen condition with TN removal efficiency of 90.8%. The effluent NH ₄ ⁺ -N, PO ₄ ^3- -P and TN concentrations were 1.0, 0.1 and 7.2 mg/L, respectively. Microbial analysis revealed that Dechloromonas (6.7%) dominated DPR process, whereas the gradually enriched Nitrosomonas (4.5%) and Candidatus Competibacter (6.8%) conducted SPNED process accompanied with sharply eliminated Nitrospirae (1.4%). Based on these findings, a novel strategy was proposed to achieve further nutrient removal in conventional treatment through integrating the DPR-SPNED process. As a result, ∼100% of extra carbon and ∼10% of oxygen consumptions would be reduced with satisfactory effluent quality.
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