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miR-23b and miR-27b are oncogenic microRNAs in breast cancer: evidence from a CRISPR/Cas9 deletion study.
- Source :
-
BMC cancer [BMC Cancer] 2019 Jun 28; Vol. 19 (1), pp. 642. Date of Electronic Publication: 2019 Jun 28. - Publication Year :
- 2019
-
Abstract
- Background: Altered expression of microRNAs (miRNAs) is known to contribute to cancer progression. miR-23b and miR-27b, encoded within the same miRNA cluster, are reported to have both tumor suppressive and oncogenic activity across human cancers, including breast cancer.<br />Methods: To clarify this dichotomous role in breast cancer, miR-23b and miR-27b were knocked out using CRISPR/Cas9 gene knockout technology, and the role of endogenous miR-23b and miR-27b was examined in a breast cancer model system in vitro and in vivo.<br />Results: Characterization of the knockout cells in vitro demonstrated that miR-23b and miR-27b are indeed oncogenic miRNAs in MCF7 breast cancer cells. miR-23b and miR-27b knockout reduced tumor growth in xenograft nude mice fed a standard diet, supporting their oncogenic role in vivo. However, when xenograft mice were provided a fish-oil diet, miR-27b depletion, but not miR-23b depletion, compromised fish-oil-induced suppression of xenograft growth, indicating a context-dependent nature of miR-27b oncogenic activity.<br />Conclusions: Our results demonstrate that miR-23b and miR-27b are primarily oncogenic in MCF7 breast cancer cells and that miR-27b may have tumor suppressive activity under certain circumstances.
- Subjects :
- Animals
Breast Neoplasms diet therapy
Breast Neoplasms pathology
CRISPR-Cas Systems
Cell Movement
Cell Proliferation
Cell Survival drug effects
Dietary Supplements
Female
Fish Oils administration & dosage
Fish Oils pharmacology
Gene Expression Regulation, Neoplastic
Gene Knockout Techniques
Humans
MCF-7 Cells
Mice, Nude
Xenograft Model Antitumor Assays
Breast Neoplasms genetics
MicroRNAs genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2407
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC cancer
- Publication Type :
- Academic Journal
- Accession number :
- 31253120
- Full Text :
- https://doi.org/10.1186/s12885-019-5839-2