Waterborne zinc pyrithione modulates immunity, biochemical, and antioxidant parameters in the blood of olive flounder.

In this study, potential immunological and hematological effects of different concentrations (0, 1, 10, and 50 μg L ^-l ) of waterborne zinc pyrithione (ZnPT) were studied in the blood of the olive flounder Paralichthys olivaceus over 30 days. Reduced alternative complement activity (ACH50) and lysozyme activity were measured in fish exposed to 10 and/or 50 μg L ^-l of ZnPT for 20 days. Decreased levels of total Ig were also observed in response to 10 and/or 50 μg L ^-l ZnPT during the exposure period. Levels of cortisol, a marker of stress, were significantly increased by 10 and 50 μg L ^-l ZnPT from day 10, and by 1 μg L ^-l exposure on day 30. The levels of red blood cells (RBCs) and white blood cells (WBCs) decreased following exposure to 10 and/or 50 μg L ^-l ZnPT, while no significant change was observed in hemoglobin level. Concentrations of total protein and albumin were significantly reduced with 50 μg L ^-l ZnPT at day 20. Alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities were significantly increased following exposure to 10 and/or 50 μg L ^-l ZnPT. Lipid peroxidation was induced by ZnPT, and higher concentrations (10 and 50 μg L ^-l ) significantly increased intracellular malondialdehyde levels during exposure. Regarding the subsequent antioxidant response, intracellular glutathione levels increased significantly in response to 10 and 50 μg L ^-l ZnPT on days 20 and 30. Similarly, catalase and superoxide dismutase activity was significantly increased in response to 10 and 50 μg L ^-l ZnPT after day 10. Taken together, changes in the studied parameters suggested the immunotoxicity of ZnPT, with modulations observed in hematological homeostasis and oxidative stress induction in the blood of olive flounder.
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