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FSH receptor binding inhibitor up-regulates ARID1A and PTEN genes associated with ovarian cancers in mice.
- Source :
-
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2019; Vol. 52 (7), pp. e8381. Date of Electronic Publication: 2019 Jun 19. - Publication Year :
- 2019
-
Abstract
- Experiments were conducted to determine if the follicle-stimulating hormone (FSH) receptor binding inhibitor (FRBI) impacts the expression levels of AT-rich interactive domain-containing protein 1A (ARID1A) and phosphatase and tensin homolog (PTEN) in ovaries and blood, as well as expressions of follicle-stimulating hormone cognate receptor (FSHR) gene and proteins. Mice in FRBI-10, FRBI-20, FRBI-30, and FRBI-40 groups were intramuscularly injected with 10, 20, 30, and 40 mg FRBI/kg, respectively, for five consecutive days. Western blotting and qRT-PCR were utilized to determine expression levels of ARID1A and PTEN proteins and mRNAs. Serum ARID1A and PTEN concentrations of the FRBI-40 group were higher than the control group (CG) and FSH group (P<0.05). FSHR mRNA levels of FRBI-20, FRBI-30, and FRBI-40 groups were lower than that of CG and FSH groups on day 15 (P<0.05 or P<0.01). Expression levels of FSHR proteins of FRBI-30 and FRBI-40 groups were lower than those of CG and FSH groups (P<0.05). Levels of ARID1A and PTEN proteins of the FRBI-30 group were greater than CG on days 20 and 30 (P<0.05). FRBI doses had significant positive correlations to levels of ARID1A and PTEN proteins. Additionally, ARID1A and PTEN had negative correlations to FSHR mRNAs and proteins. A high dose of FRBI could promote the expression levels of ARID1A and PTEN proteins in ovarian tissues. FRBI increased serum concentrations of ARID1A and PTEN. However, FRBI depressed expression levels of FSHR mRNAs and proteins in mouse ovaries.
- Subjects :
- Animals
Blotting, Western
DNA-Binding Proteins blood
Female
Mice
Nuclear Proteins metabolism
PTEN Phosphohydrolase metabolism
Phosphorylation
Real-Time Polymerase Chain Reaction
Transcription Factors
Transcriptional Activation genetics
Up-Regulation
DNA-Binding Proteins metabolism
Follicle Stimulating Hormone metabolism
Nuclear Proteins blood
Ovarian Neoplasms metabolism
PTEN Phosphohydrolase blood
Receptors, FSH antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1414-431X
- Volume :
- 52
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
- Publication Type :
- Academic Journal
- Accession number :
- 31241714
- Full Text :
- https://doi.org/10.1590/1414-431X20198381