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Fibronectin-induced ductal formation in salivary gland self-organization model.
- Source :
-
Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2019 Sep; Vol. 248 (9), pp. 813-825. Date of Electronic Publication: 2019 Jul 17. - Publication Year :
- 2019
-
Abstract
- Background: Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique-sensitive and time-consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self-organization into a well-developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real-time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning.<br />Results: On a time-scale of hours, we observed marked size and shape transformations in the developed 3D spheroid which resulted in a spatially-controlled growth differential from the canter to the periphery of the formed aggregates. Moreover, we investigated the effect of fibronectin (FN) on SMG cells self-organization using our simplified culture model. Interestingly, we discovered a novel role of FN in inducing duct-like elongation during initial stages of SMG bud formation.<br />Conclusion: This in vitro model provides an excellent tool for analyzing the intercellular dynamics during early SMG tissue development as well as revealing a novel role of FN in SMG ductal expansion.<br /> (© 2019 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Cell Culture Techniques methods
Cells, Cultured
Collagen
Drug Combinations
Laminin
Mice
Proteoglycans
Salivary Ducts cytology
Salivary Ducts enzymology
Salivary Glands cytology
Salivary Glands diagnostic imaging
Spheroids, Cellular cytology
Submandibular Gland cytology
Submandibular Gland diagnostic imaging
Fibronectins pharmacology
Organogenesis drug effects
Salivary Ducts growth & development
Salivary Glands growth & development
Submandibular Gland growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0177
- Volume :
- 248
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Developmental dynamics : an official publication of the American Association of Anatomists
- Publication Type :
- Academic Journal
- Accession number :
- 31237723
- Full Text :
- https://doi.org/10.1002/dvdy.78