Back to Search
Start Over
Emergence of phylogenetically diverse and fluoroquinolone resistant Salmonella Enteritidis as a cause of invasive nontyphoidal Salmonella disease in Ghana.
- Source :
-
PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2019 Jun 20; Vol. 13 (6), pp. e0007485. Date of Electronic Publication: 2019 Jun 20 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Background: Salmonella enterica serovar Enteritidis is a cause of both poultry- and egg-associated enterocolitis globally and bloodstream-invasive nontyphoidal Salmonella (iNTS) disease in sub-Saharan Africa (sSA). Distinct, multi-drug resistant genotypes associated with iNTS disease in sSA have recently been described, often requiring treatment with fluoroquinolone antibiotics. In industrialised countries, antimicrobial use in poultry production has led to frequent fluoroquinolone resistance amongst globally prevalent enterocolitis-associated lineages.<br />Methodology/principal Findings: Twenty seven S. Enteritidis isolates from patients with iNTS disease and two poultry isolates, collected between 2007 and 2015 in the Ashanti region of Ghana, were whole-genome sequenced. These isolates, notable for a high rate of diminished ciprofloxacin susceptibility (DCS), were placed in the phyletic context of 1,067 sequences from the Public Health England (PHE) S. Enteritidis genome database to understand whether DCS was associated with African or globally-circulating clades of S. Enteritidis. Analysis showed four of the major S. Enteritidis clades were represented, two global and two African. All thirteen DCS isolates, containing a single gyrA mutation at codon 87, belonged to a global PT4-like clade responsible for epidemics of poultry-associated enterocolitis. Apart from two DCS isolates, which clustered with PHE isolates associated with travel to Spain and Brazil, the remaining DCS isolates, including one poultry isolate, belonged to two monophyletic clusters in which gyrA 87 mutations appear to have developed within the region.<br />Conclusions/significance: Extensive phylogenetic diversity is evident amongst iNTS disease-associated S. Enteritidis in Ghana. Antimicrobial resistance profiles differed by clade, highlighting the challenges of devising empirical sepsis guidelines. The detection of fluoroquinolone resistance in phyletically-related poultry and human isolates is of major concern and surveillance and control measures within the region's burgeoning poultry industry are required to protect a human population at high risk of iNTS disease.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Adolescent
Animals
Child
Child, Preschool
Communicable Diseases, Emerging microbiology
Communicable Diseases, Emerging veterinary
Enterocolitis epidemiology
Enterocolitis microbiology
Enterocolitis veterinary
Female
Genetic Variation
Genotype
Ghana epidemiology
Humans
Infant
Male
Microbial Sensitivity Tests
Molecular Epidemiology
Phylogeny
Poultry
Salmonella Infections microbiology
Salmonella Infections, Animal microbiology
Salmonella enteritidis classification
Salmonella enteritidis genetics
Whole Genome Sequencing
Anti-Bacterial Agents pharmacology
Communicable Diseases, Emerging epidemiology
Fluoroquinolones pharmacology
Salmonella Infections epidemiology
Salmonella Infections, Animal epidemiology
Salmonella enteritidis drug effects
Salmonella enteritidis isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1935-2735
- Volume :
- 13
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PLoS neglected tropical diseases
- Publication Type :
- Academic Journal
- Accession number :
- 31220112
- Full Text :
- https://doi.org/10.1371/journal.pntd.0007485