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Transcriptome Changes during Major Developmental Transitions Accompanied with Little Alteration of DNA Methylome in Two Pleurotus Species.
- Source :
-
Genes [Genes (Basel)] 2019 Jun 17; Vol. 10 (6). Date of Electronic Publication: 2019 Jun 17. - Publication Year :
- 2019
-
Abstract
- Pleurotus tuoliensis (Pt) and P. eryngii var. eryngii (Pe) are important edible mushrooms. The epigenetic and gene expression signatures characterizing major developmental transitions in these two mushrooms remain largely unknown. Here, we report global analyses of DNA methylation and gene expression in both mushrooms across three major developmental transitions, from mycelium to primordium and to fruit body, by whole-genome bisulfite sequencing (WGBS) and RNA-seq-based transcriptome profiling. Our results revealed that in both Pt and Pe the landscapes of methylome are largely stable irrespective of genomic features, e.g., in both protein-coding genes and transposable elements (TEs), across the developmental transitions. The repressive impact of DNA methylation on expression of a small subset of genes is likely due to TE-associated effects rather than their own developmental dynamics. Global expression of gene orthologs was also broadly conserved between Pt and Pe, but discernible interspecific differences exist especially at the fruit body formation stage, and which are primarily due to differences in trans-acting factors. The methylome and transcriptome repertories we established for the two mushroom species may facilitate further studies of the epigenetic and transcriptional regulatory mechanisms underpinning gene during development in Pleurotus and related genera.
- Subjects :
- Agaricales genetics
DNA Transposable Elements genetics
Epigenomics
Gene Expression Regulation genetics
Gene Expression Regulation, Fungal genetics
Genetic Speciation
Humans
Pleurotus classification
Species Specificity
Whole Genome Sequencing
DNA Methylation genetics
Epigenome genetics
Pleurotus genetics
Transcriptome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4425
- Volume :
- 10
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Genes
- Publication Type :
- Academic Journal
- Accession number :
- 31212970
- Full Text :
- https://doi.org/10.3390/genes10060465