CM01: a facility for cryo-electron microscopy at the European Synchrotron.

Authors :: Kandiah E
Giraud T
de Maria Antolinos A
Dobias F
Effantin G
Flot D
Hons M
Schoehn G
Susini J
Svensson O
Leonard GA
Mueller-Dieckmann C
Source :: Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2019 Jun 01; Vol. 75 (Pt 6), pp. 528-535. Date of Electronic Publication: 2019 May 28.
Publication Year :: 2019
Abstract: Recent improvements in direct electron detectors, microscope technology and software provided the stimulus for a `quantum leap' in the application of cryo-electron microscopy in structural biology, and many national and international centres have since been created in order to exploit this. Here, a new facility for cryo-electron microscopy focused on single-particle reconstruction of biological macromolecules that has been commissioned at the European Synchrotron Radiation Facility (ESRF) is presented. The facility is operated by a consortium of institutes co-located on the European Photon and Neutron Campus and is managed in a similar fashion to a synchrotron X-ray beamline. It has been open to the ESRF structural biology user community since November 2017 and will remain open during the 2019 ESRF-EBS shutdown.<br /> (open access.)