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The pore structure of Clostridium perfringens epsilon toxin.
- Source :
-
Nature communications [Nat Commun] 2019 Jun 14; Vol. 10 (1), pp. 2641. Date of Electronic Publication: 2019 Jun 14. - Publication Year :
- 2019
-
Abstract
- Epsilon toxin (Etx), a potent pore forming toxin (PFT) produced by Clostridium perfringens, is responsible for the pathogenesis of enterotoxaemia of ruminants and has been suggested to play a role in multiple sclerosis in humans. Etx is a member of the aerolysin family of β-PFTs (aβ-PFTs). While the Etx soluble monomer structure was solved in 2004, Etx pore structure has remained elusive due to the difficulty of isolating the pore complex. Here we show the cryo-electron microscopy structure of Etx pore assembled on the membrane of susceptible cells. The pore structure explains important mutant phenotypes and suggests that the double β-barrel, a common feature of the aβ-PFTs, may be an important structural element in driving efficient pore formation. These insights provide the framework for the development of novel therapeutics to prevent human and animal infections, and are relevant for nano-biotechnology applications.
- Subjects :
- Animals
Bacterial Toxins genetics
Bacterial Toxins isolation & purification
Bacterial Toxins metabolism
Biotechnology methods
Cell Line
Clostridium Infections microbiology
Clostridium Infections prevention & control
Clostridium perfringens genetics
Clostridium perfringens metabolism
Clostridium perfringens pathogenicity
Cryoelectron Microscopy
Dogs
Enterotoxemia microbiology
Enterotoxemia prevention & control
Models, Molecular
Mutagenesis, Site-Directed
Nanotechnology methods
Protein Conformation, beta-Strand genetics
Protein Multimerization genetics
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Bacterial Toxins chemistry
Clostridium perfringens ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 31201325
- Full Text :
- https://doi.org/10.1038/s41467-019-10645-8