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Methods for Transposon Mutagenesis in Proteus mirabilis.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 2016, pp. 81-85. - Publication Year :
- 2019
-
Abstract
- Several methods for transposon mutagenesis have been employed for use in P. mirabilis. The first method involves the use of mini-Tn5 derivatives, which are delivered by conjugation of a suicide plasmid containing this transposon, followed by transposition into the chromosome. A second method is the use of preformed transposon/transposase complexes (transposomes), which are introduced into P. mirabilis cells by electroporation. Each of these methods will be discussed along with the advantages and disadvantages of each.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2016
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 31197711
- Full Text :
- https://doi.org/10.1007/978-1-4939-9570-7_8