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Mutation of a bHLH transcription factor allowed almond domestication.
- Source :
-
Science (New York, N.Y.) [Science] 2019 Jun 14; Vol. 364 (6445), pp. 1095-1098. - Publication Year :
- 2019
-
Abstract
- Wild almond species accumulate the bitter and toxic cyanogenic diglucoside amygdalin. Almond domestication was enabled by the selection of genotypes harboring sweet kernels. We report the completion of the almond reference genome. Map-based cloning using an F <subscript>1</subscript> population segregating for kernel taste led to the identification of a 46-kilobase gene cluster encoding five basic helix-loop-helix transcription factors, bHLH1 to bHLH5. Functional characterization demonstrated that bHLH2 controls transcription of the P450 monooxygenase-encoding genes PdCYP79D16 and PdCYP71AN24 , which are involved in the amygdalin biosynthetic pathway. A nonsynonymous point mutation (Leu to Phe) in the dimerization domain of bHLH2 prevents transcription of the two cytochrome P450 genes, resulting in the sweet kernel trait.<br /> (Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
- Subjects :
- Amino Acid Substitution
Amygdalin biosynthesis
Amygdalin metabolism
Basic Helix-Loop-Helix Transcription Factors chemistry
Cytochrome P-450 Enzyme System genetics
Leucine genetics
Multigene Family
Phenylalanine genetics
Point Mutation
Protein Conformation
Protein Multimerization genetics
Prunus dulcis metabolism
Taste
Transcription, Genetic
Amygdalin genetics
Basic Helix-Loop-Helix Transcription Factors genetics
Domestication
Gene Expression Regulation, Plant
Plant Proteins genetics
Prunus dulcis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 364
- Issue :
- 6445
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 31197015
- Full Text :
- https://doi.org/10.1126/science.aav8197