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Synaptophysin identified in metastases of neuroendocrine tumors by immunocytochemistry and immunoblotting.
- Source :
-
American journal of clinical pathology [Am J Clin Pathol] 1987 Nov; Vol. 88 (5), pp. 560-9. - Publication Year :
- 1987
-
Abstract
- Synaptophysin, an Mr 38,000 integral membrane glycoprotein of neurotransmitter vesicles, has been identified in diverse primary neuroendocrine (NE) tumors of both neural and epithelial origin (Wiedenmann and co-workers, Proc Natl Acad Sci USA 1986; 83: 3500-3504). In the present study, metastases of several types of NE tumors, including medullary thyroid carcinoma, gastrinoma, insulinoma, small (oat) cell carcinoma of the lung, gastrointestinal carcinoid, and neuroblastoma, were examined for the presence of synaptophysin by immunocytochemistry, with the use of tissue sections as well as centrifuged cell suspensions and by immunoblotting of tumor proteins. The results show that expression of synaptophysin can be maintained during formation of metastases. Therefore, the authors propose that synaptophysin antibodies be used for the positive identification of metastatic NE tumors, notably in differential diagnosis. The possible implications of these findings for tumor diagnosis are discussed.
- Subjects :
- Endocrine System Diseases classification
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Immunologic Techniques
Neoplasm Metastasis
Neoplasms classification
Neoplasms metabolism
Nervous System Neoplasms classification
Nervous System Neoplasms metabolism
Synaptophysin
Endocrine System Diseases metabolism
Membrane Proteins metabolism
Nervous System Neoplasms secondary
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9173
- Volume :
- 88
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of clinical pathology
- Publication Type :
- Academic Journal
- Accession number :
- 3118696
- Full Text :
- https://doi.org/10.1093/ajcp/88.5.560