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Chemical Composition, Antimicrobial Activity, and Antioxidant Activity of Ocotea minarum (Nees & Mart.) Mez.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2019 Apr 28; Vol. 2019, pp. 5736919. Date of Electronic Publication: 2019 Apr 28 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Ocotea minarum is a native plant from Brazil, popularly known as "canelinha" or "canela vassoura." The objective of this study was to investigate the chemical composition of the extracts of the bark and the leaves of O. minarum and to evaluate its antimicrobial and antioxidant activities. The phenolic compounds, flavonoids and tanins, were quantified with the reagents Folin-Ciocalteu, aluminium chloride, and vanillin. The chemical profile was performed by HPLC-DAD. The minimum inhibitory concentration was evaluated by the microdilution in a broth method. The antioxidant activity was measured by the capture of free radicals 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid). In addition, protection against oxidative hemolysis and generation of malondialdehyde were evaluated in human erythrocytes. The composition of the extracts included the caffeic acid, p-coumaric acid, and rosmarinic acid, besides the flavonoids quercetin and luteolin. The EEL showed bacteriostatic action of 1000 μ g/mL for all evaluated Salmonella Typhimurium, Salmonella Enteritidis, Pseudomonas aeruginosa , and Proteus mirabilis , and the EHEB had a moderate antifungal action against Candida krusei and Cryptococcus gattii (250 μ g/mL). IC <subscript>50</subscript> values of 8.19 (EEL) and 4.51 μ g/mL (EEB) in the assay with DPPH and 6.25 (EEL) and 2.87 μ g/mL (EEB) in the assay with ABTS were obtained. Up to the 3rd hour of oxidative hemolysis testing induced by AAPH, the EEB and EEL had a protective action, reducing the malondialdehyde. In conclusion, the data indicate that the O. minarum extracts can be evaluated as bioactive supplies for the development of new drugs for the prevention and treatment of diseases related to oxidative stress and microbial infections.
- Subjects :
- Benzothiazoles chemistry
Biphenyl Compounds chemistry
Candida drug effects
Cryptococcus gattii drug effects
Microbial Sensitivity Tests
Picrates chemistry
Proteus mirabilis drug effects
Pseudomonas aeruginosa drug effects
Salmonella enteritidis drug effects
Salmonella typhimurium drug effects
Sulfonic Acids chemistry
Anti-Infective Agents chemistry
Anti-Infective Agents pharmacology
Antioxidants chemistry
Antioxidants pharmacology
Ocotea chemistry
Plant Extracts chemistry
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2019
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 31182994
- Full Text :
- https://doi.org/10.1155/2019/5736919