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Amine modified magnetic polystyrene for extraction of drugs from urine samples.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2019 Sep 27; Vol. 1602, pp. 107-116. Date of Electronic Publication: 2019 Jun 04. - Publication Year :
- 2019
-
Abstract
- Polystyrene is one of the best candidates as the extracting medium due to its high stability in different media and acceptable extraction capability. However, the hydrophobic nature and low wettability of polystyrene limits its application to non-polar analytes. To resolve this limitation, in this project, amine groups were chemically attached to the surface of magnetic polystyrene. The resulting hydrophilic magnetic particles were expected to be capable of extracting both polar and non-polar analytes. Non-steroidal anti-inflammatory drugs (NSAIDs) were chosen for testing the applicability of modified magnetic polystyrene according to the importance of their analysis and also their wide polarity range. Major parameters associated with the extraction procedure were optimized using central composite design (CCD) method and pH 3, extraction time of 15 min, desorption volume of 350 μL and desorption time of 4 min were chosen as optimized values. Under these conditions, figures of merit were calculated including: linear dynamic range (0.5-1000 μg L <superscript>-1</superscript> ), linear equation and limits of detection (0.1-3 μg L <superscript>-1</superscript> ). To investigate the method precision, inter-day, intra-day and synthesis-to-synthesis relative standard deviation (RSD) were studied (<12%). All studies were conducted using blank urine samples spiked with aspirin, salicylic acid, ibuprofen, diclofenac and mefenamic acid. Naproxen was chosen as the internal standard and high-performance liquid chromatography-UV-Vis (HPLC-UV) was employed for the subsequent determination after extraction. To evaluate the applicability of the method for real sample analysis, urine samples from patients under treatment were analyzed and acceptable results were obtained. The aminated magnetic polystyrene revealed superior extraction efficiency, much higher than polystyrene before functionalization. In addition, hospital wastewater sample was tested and acceptable extraction efficiencies were obtained.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Anti-Inflammatory Agents, Non-Steroidal chemistry
Anti-Inflammatory Agents, Non-Steroidal urine
Diclofenac chemistry
Diclofenac urine
Humans
Ibuprofen chemistry
Ibuprofen urine
Limit of Detection
Reproducibility of Results
Solid Phase Extraction methods
Spectroscopy, Fourier Transform Infrared
Wastewater chemistry
Amines chemistry
Magnetics
Pharmaceutical Preparations isolation & purification
Pharmaceutical Preparations urine
Polystyrenes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1602
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 31182304
- Full Text :
- https://doi.org/10.1016/j.chroma.2019.06.007